Chromatography Products from Nacalai Tesque and Alltech distributed by Timtec

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5C18-MS-II column

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The Cosmosil 5C18-MS-II column, taking advantage of a new end capping treatment can replace the Cosmosil 5C18-MS. The silica gel is end capped with polar groups for shielding effect, based on the chemical property of the packed silica gel to draw highly polar components from the mobile phase. As a result, the new end capping provides improved peak shape for basic compounds. The absorption of the silica gel is controlled because it does not interact with N atoms, therefore suitable for the separation of chelating compounds. Another advantage is that the 5C18-MS-II has similar retention characteristics to the 5C18-MS series: the HPLC conditions for the 5C18-MS are applicable to the newly developed column, 5C18-MS-II.
Material characteristics
Silica gel high purity porous spherical silica
Average particle size 5m
Average pore size approx. 120
Specific surface area approx. 300m2/g
Stationary phase monomeric type octadecyl group
End capping treatment near-perfect treatment
Carbon content approx. 16%
Stability under alkaline condition
The durability under extremely high pH is greatly improved at the new COSMOSIL 5C18-MS-II compared with current COSMOSIL 5C18-MS. Though it is possible to use under alkaline condition for a limited time, after long exposure some deterioration may occur. For long use we recommend pH 7.5 and below.
Stability under alkaline condition
Method: After the indicated length of incubation with the extremely high pH buffer, the column was washed and the effective plate number (N) for n-amylbenzene was measured.
The figure above shows the change in N as a function of incubation time. There is hardly any observed change in MS-II. On the other hand with MS when 10 hours or more passed rapid deterioration was observed.
Alkaline solution;0.02M phosphate buffer (pH10.0)

Ordering Information
COSMOSIL 5C18-MS-II Guard Column 4.6mmI.D. 10mm 38014-31 Waters type
10mmI.D. 20mm 38016-11
COSMOSIL 5C18-MS-II Packed Column 4.6mmI.D. 50mm 38017-01
4.6mmI.D.100mm 38018-91
4.6mmI.D.150mm* 38019-81
4.6mmI.D.250mm* 38020-41
6mmI.D.150mm 38021-31
6mmI.D.250mm 38022-21
COSMOSIL 5C18-MS-II Packed Column  10mmI.D.250mm 38023-11
20mmI.D.250mm* 38024-01
COSMOSIL 5C18-MS-II Packed Column 2.0mmI.D.150mm* 38025-91
*Screw-in type column is available.



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