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COSMOSIL Ar-300 Columns

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Reversed-phase HPLC is the preferred separation method for the analysis of proteins and peptides, due to the superior resolution, high theoretical plate number, good reproducibility, ease of use and favorable cost performance.
In general, trifluoroacetic acid (TFA) is more often used as an acid mobile phase, in the analysis of proteins and peptides. However, reversed-phase HPLC using conventional silica gel shows some degradation over time in these very acidic conditions. The COSMOSIL AR-300 series packed columns have succeeded in improving acid resistance due largely to the development of a new synthesis method. It can be used for a longer time, while providing sharp peak shape even in the concentrations of 0.1% of trifluoroacetic acid.
Acid resistance
The COSMOSIL AR-300 series packed column show superior stability in the following decomposition test under the strong acidic conditions.
Capacity factor: toluene, Mobile phase :60% methanol
Types
Three alkyl and one aromatic type stationary phases are available in the COSMOSIL AR-300 series packed column. Please consult the table and applications below for selection guidance.
Alkyl type: 5C18-AR-300, 5C8-AR-300, 5C4-AR-300
Aromatic type: 5Ph-AR-300
Material characteristics

5C18-AR-300 5C8-AR-300 5C4-AR-300 5Ph-AR-300
Silica gel high purity spherical porous silica
Average particle size approx. 300
Specific surface area approx. 150m/g
Stationary phase octadecyl group octyl group butyl group phenyl group
End capping treatment near-perfect treatment
Carbon content approx. 12% approx. 7% approx. 6% approx. 7%
Semi-prep separation proteins
The sample loading capacity for protein or polypeptide separations is proportional to the cross section of column inside diameter. Using a short column, one can load a relatively large quantity of sample due to the absorption and concentration of sample inside the column. Below examples are shown for the separation using COSMOSIL 5C 18-AR-300, with 4.6mmI.D. x 150mm column, 10mmI.D. x 150mm and 10mmI.D. x 250mm columns.
Identical separation patterns are achieved by adjusting proportionally the flow rate and the sample injection volume to the cross section of the column. There is little difference between 10mmI.D. x 150mm column and 10mmI.D. x 250mm column. Thus, semi-prep separations may be achieved using a shorter column.
Ordering information
COSMOSIL 5C18-AR-300
PRODUCTNAME SIZE CODE No. END-FITTING
COSMOSIL 5C18-AR-300 Guard Column 4.6mml.D.10mm 37910-11 Waters type
10mml.D.20mm 37965-11
COSMOSIL 5C18-AR-300 Packed Column 4.6mml.D.50mm 37911-01
4.6mml.D.100mm 37912-91
4.6mml.D.150mm 37913-81
4.6mml.D.250mm 37914-71
6mml.D.150mm 37915-61
6mml.D.250mm 37916-51
10ml.D.150mm 37917-41
10mml.D.250mm 37918-31
20mml.D.150mm* 37919-21
20mml.D.250mm* 37920-81
2.0mml.D.150mm* 37991-61
5C8-AR-300
PRODUCTNAME SIZE CODE No. END-FITTING
COSMOSIL 5C8-AR-300 Guard Column 4.6mml.D.10mm 37950-91 Waters type
COSMOSIL 5C8-AR-300 Packed Column 4.6mml.D.50mm 37956-31
10mml.D.100mm 37952-71
20mml.D.150mm 37953-61
20mml.D.250mm 37954-51
5C4-AR-300
PRODUCTNAME SIZE CODE No. END-FITTING
COSMOSIL 5C4-AR-300 Guard Column 4.6mml.D.10mm 37955-41 Waters type
COSMOSIL 5C4-AR-300 Packed Column 4.6mml.D.50mm 37956-31
10mml.D.100mm 37957-21
20mml.D.150mm 37958-11
20mml.D.250mm 37959-01
10mmI.D.250mm 38047-11
20mmI.D.250mm 38048-01
5Ph-AR-300
PRODUCTNAME SIZE CODE No. END-FITTING
COSMOSIL 5Ph-AR-300 Guard Column 4.6mml.D.10mm 37960-61 Waters type
COSMOSIL 5Ph-AR-300 Packed Column 4.6mml.D.50mm 37961-51
10mml.D.100mm 379762-41
20mml.D.150mm 37963-31
20mml.D.250mm 37964-21
*Screw in type column is available.

 

 

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