Fast LC columns (4.6mm I.D.×50mm) packed with 3µm
totally porous spherical packing materials provides more than
5,000 theoretical plates. The column length is one fifth that of
250mm columns. The analysis time of Fast LC 4.6mm I.D.×50mm
column becomes one fifth that of 4.6mm I.D.×250mm column since
analysis time is in general proportional to column length. The
necessary time for the column equilibrium also becomes shorter.
Other benefits of using Fast LC columns are reduced solvent
consumption and improved detection limits.
Material characteristics
3C18-MS-II
3C18-AR-II
3SL-II
Silica gel
high purity spherical porous silica
Average particle size
3µm
Average pore size
approx. 120Å
Specific surface area
approx. 300m2/g
Stationary phase
monomeric type
polymeric type
-
End capping treatment
near-perfect treatment
-
Carbon content
approx. 16%
approx. 17%
-
Recommended operating procedures
The use of a COSMOSIL Pre-Filter (1µm) between injector and
column is highly recommended in order to prevent column clogging.
It should also be noted that the use of a guard column or a long
connecting tube may reduce column efficiency.
Comparison of theoretical plate numbers
In the example analysis of drugs using a COSMOSIL 5C18
and a COSMOSIL 3C18, the Fast LC
column is twice as efficient.
Analysis of furans
The COSMOSIL 3C18 provides the
same elution profile as the COSMOSIL 5C18-MS
does but in one fifth of the time.
