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Cosmosil GPC Columns

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High speed & high resolution
COSMOSIL non-aqueous GPC columns 5GPC-60, 5GPC-100 and 5GPC-300 can provide high resolution, high-speed analysis. GPC packing materials are 5m diameter particles of totally porous spherical silica gel. Without swelling they display excellent stability against pressure.
For GPC, all samples are eluted within t0. The t0 value for a COSMOSIL 5GPC 4.6mmI.D.x250mm column is about three minutes at a flow rate of 1.0ml/min; meaning that the analysis is completed within about three minutes. COSMOSIL 5GPC column therefore require less solvent. This is important, especially when expensive solvents such as 1,1,1,3,3,3-hexafluoro-2-propanol are used. In each molecular weight range, the GPC columns provide linear correlation between log MW and the elution volume. The large pore volume results in high peak capacity. Higher efficiencies are obtained with multiple columns. Coupled columns (GPC-60 and GPC-100, or GPC-100 and GPC-300) are frequently employed to meet the wide molecular weight range of samples.
Column characteristics
Type Particle size Pore size MW range
COSMOSIL 5GPC-60 5m 60 300-15,000
COSMOSIL 5GPC-100 5m 100 1000-50,000
COSMOSIL 5GPC-300 5m 300 17,000-300,000
Recommended solvents and optimal temperature
-Solvents
Saturated hydrocarbons and the solvents specified below can be used with the GPC column. The use of solvents and mobile phases containing water should be avoided in order to prevent deterioration of column efficiency.
Solvents from the halogen series may corrode the stainless steel tubing and fittings of the HPLC system. After using these solvents, wash the column with solvent not containing halogen ions. Column washing and changing of mobile phase should be conducted with low flow rate. Flow rate of less than 0.5ml/min for 4.6mmI.D. columns and less than 1.5ml/min for 10mmI.D. columns are recommended.
-Temperature
Run the separation at a column temperature of 50C lower than the boiling point of the mobile phase in order to prevent bubble formation.
Organic solvents for non-aqueous GPC columns
SOLVENT Physical property of the solvent Toxicity
Boiling point
C
Viscosity
(20C)cp.
Refractive index(20C) Flash PointC LD50mg/kg L(ct)50ppm
1,1,1,3,3,3-Hexafluoro-2-propanol 59 - 1.2700 - - -
Tetrahydrofuran 66 0.51(25C) 1.4070 14 hazardous 200
1,2,4-Trichlorobenzene 213 1.89(25C) 1.5717 99 hazardous 75
o- Dichlorobenzene 180 1.26 1.5515 66 500 50
Toluene 110.6 0.59 1.4961 4 hazardous 6000-200
N,N- Dimethylformamide 153 0.90 1.4280 58 hazardous 100-10
Dichloromethane 40.1 0.30 1.4242 - 3000 500
1,2-Dichloroethane 84 0.84 1.4443 13 770 50
N- Methylpyrrolidone 202 1.65 1.47 95.4 hazardous -
m- Cresol 202.8 20.8 1.544 94 hazardous 5
Benzene 80.1 0.652 1.5011 27 7000 25
Dimethyl Sulioxide 189 - 1.477 95 hazardous -
Tetrachloroethylene 121 - 1.505 - hazardous 100
o- Chlorophenol 175.5 4.11 1.5473 - 670 dangerous
CarbonTetrachloride 76.8 0.969 1.4607 - hazardous 10
Trifluoroethanol 73.6 1.20
(38C)
1.291 40.6 240 4600
Chloroform 61.7 0.58 1.446 - hazardous 50
Acetone 56.2 0.316 1.359 -17.8 4-8 500
Ordering information
PRODUCT NAME SIZE CODE No. END-FITTING
COSMOSIL 5GPC-60 Packed Column 4.6mmI.D.250mm 37830-61 Waters type
10mmI.D.250mm 37826-31
COSMOSIL 5GPC-100 Packed Column 4.6mmI.D.250mm 39732-91
10mmI.D.250mm 39744-41
COSMOSIL 5GPC-300 Packed Column 4.6mmI.D.250mm 39736-51
10mmI.D.250mm 39752-31
 
 

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