Open column chromatography is an excellent and easy technique
for large-scale preparation and purification at low cost. Normal
phase conditions are traditional in open column chromatography.
Recently several attempts have been made to apply reversed phase
technique to open column chromatography because of its numerous
advantages.
However the so far available reversed phase packing materials
could not perform well over 30-50% water concentration in the
mobile phase.
The COSMOSIL C18-OPN is
not subject to such restrictions and the use of 100% aqueous
effluents results in excellent separation. The COSMOSIL C18-OPN
is a new type of reversed phase C18
packing material designed for open column chromatography.
Characteristics
The COSMOSIL C18-0PN packing
material is coated with hydrophilic groups to improve the affinity
for highly polar effluents. The hydrophobic groups in the pore of
the packing material provide superior retention characteristics in
reversed phase partition chromatography.
Material characteristics
75C18-OPN
140C18-OPN
Silica gel
totally porous spherical silica
Average particle size
75µm
140µm
Average pore
size
approx. 120Å
Specific
surface area
approx.
300m2/g
Stationary
phase
octadecyl group
Application
open column
chromatography,s flash column chromatography
Reproducibility and washing methods
Wash the COSMOSIL C18-OPN
packing material with tetrahydrofuran, chloroform or other
solvents that can remove impurities absorbed onto the packing
material. This packing material has excellent reproducibility and
can be used repeatedly.
"CAUTION"
Do not wash with basic solvents of > pH 7 which will dissolve
the silica gel or < pH 2 which will cleave the C18
stationary phase. Dry the packing material at 50°C.
Influence of particle size
Comparison table of 75µm and 140µm
