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Cosmosil and Cosmogel HPLC columns and packing materials

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Specialty Columns PYE, NPE column

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Reversed-phase HPLC columns have been widely used due to their superior resolution, high theoretical plate number, ease of use and good cost performance.
Octadecyl silica gel (C18, ODS) is the most commonly used by chromatographers. In reversed phase HPLC columns, hydrophobic interaction is the dominant separation mode. For some substances similar in hydrophobicity, recognition capability may be poor, resulting in insufficient separation.
The COSMOSIL PYE and NPE columns utilize various interactions based on electrons of the stationary phase. The PYE and NPE columns can be widely used for the separation of substances similar in structure.


Characteristics
The COSMOSIL PYE column is a reversed phase column. Its stationary phase is planar pyrene or 2-(1-pyrenyl)ethyl groups bonded chemically to the silica gel. Utilizing - interactions originating from the pyrene ring, the PYE column separates substances similar in structure.
The COSMOSIL NPE column is also a reversed phase column, which contains nitrophenylethyl groups chemically bonded to the silica gel. The NPE column provides unique retention characteristics somewhat different from the PYE column, utilizing both dipole-dipole and - interactions.
Stationary phases and interactions
Hydrophobic interaction
- interaction
Charge transfer interaction
Stereo selectivity
PYE STATIONARY PHASE
Hydrophobic interaction
- interaction
Charge transfer interaction
Dipole-dipole interaction
NPE STATIONARY PHASE
Material characteristics
  COSMOSIL 5PYE COSMOSIL 5NPE
Silica gel high purity spherical porous silica
Average particle size 5m
Average pore size approx. 120
Specific surface area approx. 300m/g
Stationary phase 2-(1-pyrenyl)ethyl group nitrophenylethyl group
End capping treatment near-perfect treatment
Carbon content approx. 18% approx. 9%
Separation of PAHs
C18 columns have been commonly used for the separation of polycyclic aromatic hydrocarbons. However, their resolution for the separation of isomers is limited. Due to the electrostatic interaction between the sample ( eg. benz[α]anthracene and chrysene) and the pyrene ring in the stationary phase, the COSMOSIL PYE packed column can achieve the separation of aromatic isomers.
 
Ordering information

COSMOSIL 5PYE
PRODUCT NAME SIZE CODE No. END-FITTING
COSMOSIL 5PYE Guard Column 4.6mmI.D.10mm 37903-11 Waters type
COSMOSIL 5PYE Packed Column 4.6mmI.D.150mm 37837-91
4.6mmI.D.50mm 38043-51
4.6mmI.D.250mm 37989-11
10mmI.D.250mm 37996-11
20mmI.D.250mm 38044-41
COSMOSIL 5NPE
PRODUCT NAME SIZE CODE No. END-FITTING
COSMOSIL 5NPE Guard Column 4.6mmI.D.10mm 37904-01 Waters type
10mmI.D.20mm 38045-31
COSMOSIL 5NPE Packed Column 4.6mmI.D.150mm 37902-21
4.6mmI.D.250mm 37990-71
20mmI.D.250mm 38046-21

      
 

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